Saturday, February 27, 2010

FINAL REPORT OF THE TESTING OF THE BELGIAN (VERMONT) SHEEP February 27, 2010

Veterinary Laboratories Agency - Weybridge
New Haw, Addlestone, Surrey KT15 3NB United Kingdom
Telephone +44 (0)1932 341111 Facsimile +44 (0)1932 347046 '
Web site http://www.vla.gov.uk

Veterinary Laboratories Agency

Your ref: MPL-6197-7-37

Our ref: FT1294

This is the FINAL report for contract MPL-6197-7-37 The testing of the Belgian (Vermont) sheep.

Background

Brain homogenate (10% in normal saline) from each case was inoculated intracerebralty into panels of 20 Rlll and 20 Tg338 mice.

FT1294/0001 (Sample 4677) was inoculated into mice on the 14/12/06

FT1294/0011 (Sample 4703) was inoculated into mice on the 20/12/06

Method

The brain from each mouse was examined histologically for any evidence of TSE-related vacuolation, and immunolabelled using anti-PrP antibody Rb486 as described elsewhere1, All slide interpretation was undertaken blind with regard to the clinical status of the mouse, or the source of the inoculum.

Final bioassay results

FT1294/0001 (Sample 4677)

Tg338 mice - All 20 mice are negative by histopathology, and immunohistochemistry

Rlll mice - All 20 mice are negative negative by histopathology, and immunohistochemistry

FT1294/0011 (Sample 4703)

Tg338 mice - All 20 mice are negative by histopathology, and immunohistochemistry

Rlll mice - All 20 mice are negative by histopathology, and immunohrstochernistry

The survival times for these mice can be seen in the figures below. Additional data sets from positive and negative inocula (J Spiropoulos, pers. comm.) have been included for comparison._

1 Beck KE, Chaplin M, Stack M: Sallis RE, Simonini S, Lockey R, Spiropoulos J. Lesion Profiling at Primary Isolation in Rlll Mice Is Insufficient in Distinguishing BSE from Classical Scrapie. Brain Pathol. 2009 epub ahead of print

FINAL report for contract MPL-6197-7-37

VLA is an Executive Agency of the Department for Environment, Food and Rural Affairs (Defra)


snip... (2 pages of charts and graphs of survival and comparison of tg338 data NOT included here...TSS)




CONCLUSION

These mice have survived for long enough to have demonstrated the presence of classical scrapie, atypical scrapie, or ovine BSE if any of these strains was present in the inoculum.

Both samples are negative by bioassay.

Dr. Marion M Simmons

22nd October 2009





February 27, 2010, INVESTIGATION OF MAD SHEEP OF MAD RIVER VALLEY COMPLETE. THEY WERE NOT INFECTED WITH ANY TSE. ...TSS


Greetings again BSE list members,

The investigation of the Mad Sheep of Mad River Valley may be complete now, but, I still have questions.


PLEASE SEE MY FINAL FOIA HERE ;


Monday, September 1, 2008
RE-FOIA OF DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E. (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [No. 00-072-1] September 1, 2008

Greetings again BSE-L members,

I had a pleasant surprise this past Saturday. I got an unexpected package from O.I.G. on my old F.O.I.A. request, of the final test results of the infamous mad sheep of mad river valley. IF you all remember, back on Thu, 24 Apr 2008 15:00:20 -0500 I wrote ;

Greetings,

With great disgust, I must report, that after years and years of wrangling over the infamous mad sheep of mad river valley, I have failed in getting an official answer via FOIA on the outcome of the TSE testing of those imported Belgium sheep. The USA Government refuses to tell the public, exactly what the testing outcome was, and in doing so, shows just how corrupt this administration has been. and the excuse given in their answer to my final appeal, which they have now officially denied, was bizarre to say the least ;

"I am denying your FOIA appeal. This is the final agency decision. You may seek judicial review of this decision in the United States district court for the judicial district in which you reside or have your principal place of business or in the District of Columbia, pursuant to 5 U.S.C. & 552(a)(4)(B)."

FOIA OF DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E. (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [Docket No. 00-072-1] ...snip...end...TSS

NOW, out of the wild blue, AFTER them telling me they denied my FOIA appeal for the final time, any further action would have to be judicial review in the United States district court, I get 25+ pages, a lot of redacted names, etc, but this is the first time they sent me anything about this in the 6 years of waiting for my FOIA request. IT will take me a long time to get this online due to the fact you cannot hardly read it, very poor quality and eligibility of text. BUT, the just of it is, somebody (REDACTED) screwed those tests up. I will work to get all the data online next week or so, but it is odd how much they were concerned for human and animal health from an atypical scrapie of foreign origin back then, but yet when we document it here in the USA, you don't hear a word about it. it's a completely different story.

IN SHORT ;

August 15, 2000

OIG case # NY-3399-56 REDACTED, VT

''Enclosed is OIG's notification that they have scheduled an investigation of the following individual. REDACTED is alleged to have provided possibly inaccurate test results involving diseased sheep. However, because the results were determined to be inconclusive, no actual violation was actually committed.''

snip...

PLEASE SEE FULL TEXT HERE ;


http://foiamadsheepmadrivervalley.blogspot.com/




Subject: RE-FOIA OF DECLARATION OF EXTRAORDINARY EMERGENCY BECAUSE OF AN ATYPICAL T.S.E. (PRION DISEASE) OF FOREIGN ORIGIN IN THE UNITED STATES [Docket No. 00-072-1]

From: TERRY SINGELTARY

Reply-To: Bovine Spongiform Encephalopathy

Date: Mon, 1 Sep 2008 13:30:02 -0500

Content-Type: text/plain


https://lists.aegee.org/




A FEW THINGS TO PONDER ;


again, which lab and test were used in the USA, and whom screwed those test up ? and why ?

Was it the rubenstein lab staten Island (and where is that at) ?

of which I have heard rumours of mice running around that lab loose, to a lab tech. getting needle stick from CJD, to they had to secretly shut it down, because they were not suppose to be working on CJD in the first place ?

OR, was it the Schmeer test ?

any follow ups on that would be much appreciated.


an old report here off Tom's page ;


http://www.mad-cow.org/00/jul00_dont_eat_sheep.html#said



Where and who is Staten Island?


http://www.mad-cow.org/00/jul00_dont_eat_sheep.html#bbb





maybe this case is not closed yet ??? something still smells fishy to me. this just gets better and better.



plus, in the end, it really did not matter, the Nor-98 was already here. ...TSS


Thursday, January 07, 2010

Scrapie and Nor-98 Scrapie November 2009 Monthly Report Fiscal Year 2010 and FISCAL YEAR 2008

http://scrapie-usa.blogspot.com/2010/01/scrapie-and-nor-98-scrapie-november.html






Monday, December 14, 2009

Similarities between Forms of Sheep Scrapie and Creutzfeldt-Jakob Disease Are Encoded by Distinct Prion Types


http://nor-98.blogspot.com/2009/12/similarities-between-forms-of-sheep.html




Terry S. Singeltary Sr.
P.O. Box 42
Bacliff, Texas USA 77518

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